Alvin and the Chipmunks: The Squeakquel is a 2009 American live-action/animated jukebox musical comedy film directed by Betty Thomas and written by Jon Vitti and the writing team of Jonathan Aibel and Glenn Berger, based on the characters Alvin and the Chipmunks created by Ross Bagdasarian Sr. and the Chipettes created by Janice Karman. It is the second installment in the live-action Alvin and the Chipmunks film series and the sequel to Alvin and the Chipmunks (2007). The film stars Zachary Levi, David Cross and Jason Lee. Justin Long, Matthew Gray Gubler and Jesse McCartney return to voice the Chipmunks from the previous film. Christina Applegate, Anna Faris and Amy Poehler voice the new characters, the Chipettes. The film sees the Chipmunks entering high school and being under the care of Dave Seville's cousin, Toby, while Ian Hawke recruits the Chipettes to restore his career.

Alvin and the Chipmunks: The Squeakquel was released in the United States on December 23, 2009, by 20th Century Fox. The film received generally negative reviews from critics, who criticized the over-reliance of slapstick humor. However, it grossed over $443 million worldwide against a $70 million budget. Two sequels were later released: Alvin and the Chipmunks: Chipwrecked in 2011 and Alvin and the Chipmunks: The Road Chip in 2015.