Madame Web is a 2024 American superhero film based on Marvel Comics featuring the character of the same name. Produced by Columbia Pictures and Di Bonaventura Pictures in association with Marvel Entertainment and TSG Entertainment, and distributed by Sony Pictures Releasing, it is the fourth film in Sony's Spider-Man Universe (SSU). The film was directed by S. J. Clarkson from a screenplay she co-wrote with Claire Parker and the writing team of Matt Sazama and Burk Sharpless. It stars Dakota Johnson in the title role, alongside Sydney Sweeney, Celeste O'Connor, Isabela Merced, Tahar Rahim, Mike Epps, Emma Roberts, and Adam Scott. In the film, Cassie Webb (Johnson) confronts her past while trying to save three young women and their futures from Ezekiel Sims (Rahim).

Sony began development on a Madame Web film for its shared universe by September 2019, with Sazama and Sharpless hired to write the script. Clarkson joined as director in May 2020, making the film her feature film directorial debut, while Johnson was cast in early 2022. This was followed by additional castings in the following months, particularly for the three Spider-Women characters. Madame Web is a suspense thriller that depicts the titular character's origin story in a standalone universe with an approach that was more grounded and gritty compared to many previously released superhero films. Filming began in mid-July 2022 and wrapped before the end of the year, occurring throughout Massachusetts, including Boston, and in New York City. Clarkson and Parker's involvement as writers was revealed in November 2023.

Madame Web premiered at the Regency Village Theatre in Westwood, Los Angeles on February 12, 2024, and was released in the United States on February 14. The film received negative reviews from critics.