Hellboy: Blood and Iron is the second in the Hellboy Animated series (the first being Hellboy: Sword of Storms), written by Tad Stones and Mike Mignola. It first aired on March 10, 2007 on Cartoon Network, and aired again on July 19, 2008 to promote the release of Hellboy II: The Golden Army, and was released on DVD by Anchor Bay Entertainment on June 12, 2007. The film's storyline is based in part upon the Hellboy: Wake the Devil storyline from the original comics.The title is a reference to Otto von Bismarck's famous "Blood and Iron speech."

Also included on the DVD is the short film Iron Shoes, which is based on the Hellboy story of the same name. The Iron Shoes demon is voiced by Dan Castellaneta.